FastQCFastQC Report
Fri 29 Apr 2016
ERR1307218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806678
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA44840.2481903250053413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38470.21293224359847188No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT35480.19638253191769645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.19483272614157032No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT33870.18747114870497122No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA33810.18713904746722992No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA33800.1870836972609397No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC27820.15398427389938882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23520.13018368519459472No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT21180.1172317369226835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.031.9762951
CTTATAG8900.025.9831472
GCTTATA9400.024.7978731
TTATAGC9900.024.1060623
GCGACTT8950.023.77094818
AAGCGAC9400.022.82978816
CGACTTA9300.021.8817219
AGCGACT9850.021.78680217
GTATCAA34250.021.6598552
ATAGCAA10450.021.2440195
TATAGCA11550.021.1428574
CAAGCGA10400.020.63461515
TATGCCG555.14466E-420.18181833
TCAAGCG10850.020.11981614
AGCAATT10650.019.9765267
GCAATTT10600.019.8962278
TCGCTAA2250.019.73333414
CTTATAC11400.019.14912437
TTAGTAC609.2396064E-418.53
TCTTATA20250.018.0888937
Produced by FastQC (version 0.11.5)