FastQCFastQC Report
Fri 29 Apr 2016
ERR1307217.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307217.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169031
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67700.13097232343934484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.1302565219670766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42500.031.5588251
CTTATAC43450.020.5224437
GTATCAA66800.019.9678132
GCTTATA8900.016.6292151
CTTATAG8600.016.5639532
ATACGTA803.3854283E-416.18756
TCTTATA74550.015.80751137
CGACTTA7500.015.53999919
TCCTATA4400.014.7159092
TTAGCGA3550.014.59154927
TTTAGCG3050.014.55737726
AAGCGAC8750.014.16571416
GCGACTT8500.014.14705918
CTACACT7300.013.9383564
CGTCTAA4400.013.8751
TTATAGC10950.013.8538813
GTATAGA8550.013.8479531
GTATTAG12850.013.8210121
ATCAACG97850.013.574864
TATCAAC100650.013.3994053
Produced by FastQC (version 0.11.5)