FastQCFastQC Report
Fri 29 Apr 2016
ERR1307216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383225
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA46540.1952816037092595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43650.18315517838223416No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT42960.18025994188547032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.17937878295167264No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT40970.1719099119890065No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA33610.14102738935685888No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC29850.12525044844695737No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT28100.11790745733197662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27510.1154318203274974No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA25870.10855038865402973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.032.888891
CTTATAG7150.021.9930082
GCTTATA7200.021.8402771
GTATCAA43950.021.130832
CTTATAC22250.020.12134737
CGACTTA7150.019.40559419
GCGACTT7500.018.99333218
AAGCGAC7700.018.98051816
AATATCG609.2407817E-418.55
TATTACG609.2407817E-418.52
TATACGT500.00703704518.54
TTATAGC8450.018.1715973
TATAGCA9400.017.7127674
AGCGACT8850.017.14124517
TAGCAAT9600.016.5729186
GATCGGA904.4491808E-516.44444511
GCAATTT9450.016.2486788
ATAGCAA9350.016.2245985
CAAGCGA9500.015.773684515
CTCGCTA1659.749783E-1015.6969713
Produced by FastQC (version 0.11.5)