FastQCFastQC Report
Fri 29 Apr 2016
ERR1307215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142344
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT429800.6997328707086415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT402640.6555152235042518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293110.4771956764388318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.14391899900103283No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA73050.11892853933286707No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT69800.11363739966371145No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT63350.10313652247415643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90350.033.130051
GTATCAA149800.019.8955292
CTTATAG16600.019.1686742
CGACTTA14250.018.69473619
GCTTATA17400.018.6063231
GCGACTT15700.017.55732518
CTTATAC38250.017.31503337
TTATAGC19600.017.1785723
AAGCGAC16300.016.79754616
AGCGACT17600.015.97727317
TCTTATA59000.014.98813637
TATAGCA23300.014.847644
ATAGCAA22750.014.149455
CAAGCGA19750.013.95696315
GCAATTT22450.013.9265048
TAGACCG1907.1449904E-913.6315795
TTAGACT7600.013.6315794
CTACACT9650.013.6113984
CTAATCG3950.013.58227826
ATCGATA5700.013.30701723
Produced by FastQC (version 0.11.5)