FastQCFastQC Report
Fri 29 Apr 2016
ERR1307213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6745594
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT455200.6748108469024373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT442350.6557613754993259No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293810.43555838077417647No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA117490.174172949039032No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT100150.1484672810133548No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT97230.14413852953498238No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA94070.13945399026386707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93030.137912243161981No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA83310.12350283755589203No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC77830.11537901628826165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106000.033.2301861
GCTTATA21450.022.1655031
CTTATAG22450.021.0957682
GTATCAA168800.020.856342
AAGCGAC21950.019.8906616
TTATAGC24200.019.7995873
GCGACTT21300.019.71596118
CTTATAC44950.019.549537
CGACTTA21250.019.50117519
AGCGACT23200.018.65948517
TATAGCA28250.017.0265484
ATAGCAA26950.016.9554735
CAAGCGA27400.015.93430615
TCTTATA73650.015.67413437
TCTATAC7500.015.2933323
GCAATTT28150.015.2468928
TAGCAAT30500.015.1032796
CCTATAC10150.014.0344833
CTAGTAC5950.013.9915963
ATCAACG257050.013.6815814
Produced by FastQC (version 0.11.5)