FastQCFastQC Report
Fri 29 Apr 2016
ERR1307212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8999808
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT345090.38344151341895294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT343130.38126368918092474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT236060.26229448450455833No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA130760.1452919884513092No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT110070.12230260912232795No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT104800.11644692864558888No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA97870.10874676437541779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108600.032.7412531
GTATCAA171050.020.7009642
CTTATAC64650.020.11678337
GCTTATA22550.019.6075381
CTTATAG21550.019.4013922
TTATAGC25000.017.3163
CGACTTA21350.016.98360619
TCTTATA109850.016.33591337
GCGACTT23400.016.20726618
AAGCGAC23750.015.65684116
TATACCG5800.015.3103466
TTAGCGT6150.014.4390244
TATAGCA33700.014.0534134
AGCGACT27750.013.66666717
TAATCGA8150.013.61963221
GTATAGA12100.013.6074381
ATAGCAA30850.013.5526735
ATCAACG262650.013.5166584
GTATTAG18700.013.4545451
TAGCGTA2750.013.4545455
Produced by FastQC (version 0.11.5)