FastQCFastQC Report
Fri 29 Apr 2016
ERR1307211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5231166
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349600.6683022484853282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT349050.6672508576481802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238070.4550993029087588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81900.15656165374985231No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA73930.1413260447097263No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT69390.13264729125399577No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT65050.12435086173904632No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA64260.12284068217296105No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA59640.11400899914091811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78050.032.875721
GCTTATA16550.022.2447131
CTTATAG17150.021.1428572
CGACTTA15800.020.60759519
GTATCAA125100.020.452042
AAGCGAC16600.020.06024216
GCGACTT16200.019.98456818
CTTATAC48700.019.6396337
AGCGACT17650.018.9716717
TTATAGC20000.018.77753
CAAGCGA19150.017.4856415
ATAGCAA21150.016.8817965
TATACCG3000.016.656
TATAGCA23950.016.3757824
TCAAGCG21400.015.56074714
TAGCAAT23600.015.3644076
GCAATTT22850.014.8971558
AGCAATT23900.014.55237
TCTTATA75050.014.54363837
GTATAGG8400.014.3154751
Produced by FastQC (version 0.11.5)