FastQCFastQC Report
Fri 29 Apr 2016
ERR1307210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7098840
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT406080.5720371215578883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401580.5656980577108373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT271510.3824709389139634No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA128830.18148035453679756No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT112420.15836390170788467No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT107580.15154588637016753No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA104770.14758749316789785No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC85640.12063942841365632No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA85040.11979421990071616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83410.11749807010722879No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT72600.10227023006575724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100650.032.404871
CTTATAG23350.022.2633822
GCTTATA23900.021.9058591
GTATCAA159400.020.42662
TTATAGC27000.019.3222243
CTTATAC53500.019.29532837
GCGACTT23150.019.17926618
CGACTTA23050.019.0216919
AAGCGAC25100.018.13147416
TATAGCA30700.017.535834
ATAGCAA28950.017.5094995
AGCGACT26300.017.23383917
CAAGCGA28300.016.27738615
TAGCAAT33250.015.5233096
TCTTATA85250.015.25571837
GCAATTT31200.015.2387838
AGCAATT34000.014.63676557
TCAAGCG32100.014.408114
GTATTAG16100.014.0186341
TAAGCAG22150.013.9480815
Produced by FastQC (version 0.11.5)