FastQCFastQC Report
Fri 29 Apr 2016
ERR1307209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6756824
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT517310.7656111806375303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT506950.7502785332280373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT335780.49694945435903015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106720.15794402814103195No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA104750.15502845715679436No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT91440.13532985319730098No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT88060.13032750298069032No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA83320.12331237279526594No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA71960.10649974011458638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117350.033.358331
GCTTATA19250.022.0077921
CTTATAG19300.021.4715022
GTATCAA184650.021.2201482
CTTATAC61350.020.14343837
GCGACTT19750.019.95189918
TTATAGC21650.019.3972283
AAGCGAC20400.019.31617516
CGACTTA20250.018.91111219
AGCGACT22400.017.67410917
CAAGCGA24200.016.74173515
TATAGCA26450.016.6465034
ATAGCAA26350.015.2352945
ATACCGT2500.014.7999996
TCTTATA93050.014.69263837
TCAAGCG28450.014.305799514
GCAATTT26700.014.2734098
TCTATAC7950.014.1949693
TAGCAAT29400.014.0952396
TAAGCAG21900.013.7694075
Produced by FastQC (version 0.11.5)