FastQCFastQC Report
Fri 29 Apr 2016
ERR1307208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1307208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9391135
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT555130.591121307488392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT548110.5836461726937159No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT371700.39579880387194943No Hit
GTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCCTA184860.19684521625980247No Hit
TATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATTT154580.16460204224515992No Hit
GGTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACAT153010.162930252839513No Hit
GAGTACGGGTAGGCGTCATTTACTCACACATTTTATGTCGATA127500.13576633708279137No Hit
GTGTTGTAGAGTATCGACATAAAATGTGTGAGTAAATGACGCC116280.12381889941950573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113750.12112486935817662No Hit
GCTTATAGCAATTTCAAGCGACTTAAATGCATATGGTGTTGTA108980.11604561110025573No Hit
GTATCAACGCAGAGTACGGGTAGGCGTCATTTACTCACACATT105630.11247841714553139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140000.032.996071
GCTTATA30050.022.1014961
CTTATAG30300.021.491752
GTATCAA218500.021.0739142
CTTATAC76350.020.45055637
GCGACTT29400.020.13605518
AAGCGAC30650.019.85807416
CGACTTA29850.019.70854219
TTATAGC35200.018.9730113
AGCGACT33400.018.33383217
TATAGCA40000.017.11254
ATAGCAA39400.016.3870545
CAAGCGA37850.016.2760915
TAGCAAT38650.016.1785266
ACGCGTA700.002594349715.85714311
TCTTATA127150.015.49547837
TCGCTAA4550.015.04395614
GCAATTT40050.014.9662918
AGCAATT42450.014.4687887
TCAAGCG42900.014.40326414
Produced by FastQC (version 0.11.5)