FastQCFastQC Report
Fri 29 Apr 2016
ERR1042871.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042871.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276790
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140241.0983795299148646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103620.8115665066299078No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49640.3887875061678115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48820.38236515010299266No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28770.22533071217663045No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17400.136279262838838No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.12296462221665269No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG12800.10025141174351303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAT250.005496568529.615
GTAGCAC1059.822543E-1021.1428573
GGTATCA39450.021.0088711
CGAACGC555.143523E-420.18181830
CTACGAA609.23758E-418.527
GTAAGAC1700.018.53
AAGACGG6950.018.1007215
CGCAAGA6900.017.6956522
TCGCGTA4500.017.6777789
CAAGACG7700.017.5389634
ACGGACC6750.017.2666688
TACGAAC650.001580063817.07692328
CGGACCA6950.017.0359739
GACGGAC7000.016.9142867
GTATCAA50700.016.4566082
GCGTAAC4950.016.44444511
GTCGCGT4900.016.2346948
CGCGTAA5000.015.9110
GAGCGAA7450.015.89261916
AACGCAC700.002592705215.85714232
Produced by FastQC (version 0.11.5)