FastQCFastQC Report
Fri 29 Apr 2016
ERR1042870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7156079
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182360.2548322901410116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT181590.253756281896832No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA143660.20075239527120925No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC134090.18737914995069227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130590.18248820338623986No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT117100.16363709791353617No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA91900.12842228264947886No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG83320.11643247650004983No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG77110.10775453988140712No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT76860.10740518655537482No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC76150.10641302310944305No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG73390.1025561623900463No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG73130.10219283493097268No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG72580.10142425761370158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73400.019.2057231
CGCAATA18650.015.57372636
TCTAGCG18550.015.55795128
CAAGACG31100.015.2282964
AAGACGG31400.015.141725
CTAGCGG18850.015.114058529
ACGGACC30100.015.0581418
GACGGAC30900.014.4886727
TCGTTTA22000.014.12727230
CGAGCCG22950.014.10675415
AGACGGA32800.013.9878046
CGCAAGA32900.013.9452882
GCGCAAT21000.013.91904835
GCGCAAG34200.013.7397661
GTATCAA102300.013.7077222
CGGACCA33200.013.6521089
TAAACGC10050.013.4378128
TAGCGGC21450.013.36829830
GTAAACG9800.013.21428527
TAGTACG2001.4664693E-812.9500014
Produced by FastQC (version 0.11.5)