FastQCFastQC Report
Fri 29 Apr 2016
ERR1042869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6494288
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT158470.2440144323750348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148010.2279079708198959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105720.1627892079932396No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102440.15773861584210616No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA85160.13113061816784227No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC80360.12373950770276897No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT78730.12122960977400449No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC72700.11194452725225613No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC72210.11119001805894657No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68010.10472279640200742No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA65920.10150458372034007No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG65030.10013414865494108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG24100.017.9626565
TCTAGCG15900.017.68553428
GACGGAC24250.017.088667
CTAGCGG16600.017.05120529
ACGGACC24500.016.7632668
CGCAAGA26100.016.2318022
CGCAATA18100.015.63812236
AGACGGA26800.015.6007466
CGAGCCG22950.015.39651315
CAAGACG28700.015.3414634
CGGACCA26850.015.1582879
GCGCAAG28750.014.9930441
CGGTCCA21250.014.45176510
GCGCAAT20100.014.0820935
TAGCGGC20850.013.84172730
TCGTTTA17250.013.72753630
TAGACCG1356.577766E-613.7037035
GCAAGAC33150.013.67273
CCGGTCC22300.013.6053819
GCAATAC20950.013.59904637
Produced by FastQC (version 0.11.5)