FastQCFastQC Report
Fri 29 Apr 2016
ERR1042868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5970311
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT535090.8962514683070948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT434210.7272820461111658No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC414580.6944026868952052No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG356670.5974060647761901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334510.5602890703683611No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA234100.3921068768444391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186880.3130155196270345No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG160370.26861247261658566No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG136000.22779382849570148No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG132620.22213248187573478No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT126050.21112803001384683No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC111590.18690818618996566No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT109860.18401051469513063No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC107420.17992362541917833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA93750.15702699574611775No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT83990.14067943864230859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT80840.135403331585239No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA80620.1350348415685548No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC75810.12697830984014066No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC75770.126911311655289No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA72070.12071397955650887No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG70860.11868728446474563No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC67890.11371266923950864No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66620.11158547687046788No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG64930.10875480356048453No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.10821881808167112No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA64580.10816856944303235No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT63980.10716359667025722No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA63650.10661086164523087No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC62870.10530439704062318No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60750.10175349324348429No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG60130.10071502137828331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG26850.017.3631295
CAAGACG28000.017.0464294
ACGGACC27450.016.377058
GACGGAC27650.016.3254977
AGACGGA29400.015.7942176
CGCAAGA29850.015.5561132
CGGACCA29550.015.2758049
CGAGCCG22700.015.07709215
TCTAGAT17100.015.0380122
TTCGGGC11050.014.90045235
GCGCAAG31600.014.8117081
CCGAGCC27800.014.7733814
GCAAGAC33400.014.5673653
TCTAGCG17650.014.35977328
TAAACGC11450.014.2183428
GTAAACG11200.014.20535727
AGAGCGA32850.014.19178215
TACCGTC19650.014.1221377
CGCAATA18000.014.08055636
TATACTG6050.014.0661165
Produced by FastQC (version 0.11.5)