FastQCFastQC Report
Fri 29 Apr 2016
ERR1042867.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042867.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6729459
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360450.5356299815482938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT353270.5249604760204349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263360.39135389635333245No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC260080.3864798046915807No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG226870.33712962661634466No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA143150.21272140895724306No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT121550.18062373216034155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118800.17653722238295827No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA118680.17635890195630882No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC104490.15527251150501103No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG103960.1544849296206426No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG86030.12784088587210354No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA82490.12258043328594469No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80170.11913290503738859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT80030.1189248645396309No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG79270.11779550183751769No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT78210.11622033806878086No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG74800.11115306594482559No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC73210.10879032029172034No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG71390.10608579382087029No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC71040.10556569257647605No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA69970.1039756687721851No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC69550.10335154727891202No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC68330.10153862294130925No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG68300.1014940428346469No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG68300.1014940428346469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG26200.018.5706125
ACGGACC28100.016.919938
GACGGAC28000.016.9142867
CGGACCA30100.015.8571439
CAAGACG32250.015.7751944
CGCAAGA30500.015.7704922
AGACGGA31150.015.6195836
TCTAGCG17650.015.51274828
GCGCAAG31850.015.4505491
CGAGCCG22200.015.33333315
TAGACCG750.00410716114.85
CTAGCGG18500.014.829
CTAGTAC3900.014.7051293
CGGTCCA20100.014.17412910
CGCAATA19600.014.15816336
CGCGAAA1051.6575168E-414.09523815
CAGAGCG34550.014.08248914
GCGAAAG34000.014.03823618
TACCGTC22050.014.0113377
ATACCGT23750.013.7873686
Produced by FastQC (version 0.11.5)