FastQCFastQC Report
Fri 29 Apr 2016
ERR1042866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159040
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT136360.19047246558197747No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA107100.14960106382978725No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC94650.13221046397282318No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT89790.12542184426962275No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC87450.12215324959771141No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA86380.12065863579474342No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG86300.12054688896835329No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG86030.1201697434292866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85310.11916402199177542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC85250.11908021187198284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84290.11773924995530126No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG83280.11632844627212588No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA82480.11521097800822457No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG75240.10509789021991775No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG75080.10487439656713748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA609.2432514E-418.513
AAGACGG32750.017.5114525
CGCAAGA33950.016.5110452
ACGGACC34400.016.1875028
GACGGAC34800.015.9482757
CTAGCGG21750.015.4804629
TATACTG8250.015.4727265
AGACGGA36200.015.3314916
CGGACCA36350.015.319129
CAAGACG38550.015.1647214
TCTAGCG22250.015.04943828
GCGCAAG38550.014.9247721
CGCAATA22650.014.78366436
GCGAAAG38300.014.39425718
AGCGAAA38700.014.19767417
CGAAAGC39000.014.04102519
GAGCGAA39400.013.94543116
CAGAGCG40350.013.93804214
GTAAACG12200.013.79918127
GCAAGAC44500.013.7606743
Produced by FastQC (version 0.11.5)