FastQCFastQC Report
Fri 29 Apr 2016
ERR1042865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296238
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339430.5390996973113151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282620.4488712148428951No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC220700.3505267748773156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT213770.3395202023811679No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG195310.3102011073914296No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA118650.18844586243404396No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT111990.17786811743774616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103360.16416151994254347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG86520.1374153899519046No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT82440.13093532995417265No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA78590.12482056745631281No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG73690.11703814245903664No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC72290.11481459245981489No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA70400.11181279996086552No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG69610.11055808246130468No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT67280.10685745996259989No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67240.10679392996262212No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA65610.10420508246352822No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG63600.10101269996464556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG27650.018.2658235
CAAGACG28500.017.9807014
ACGGACC28250.017.6159298
GACGGAC28950.016.8704667
CGCAAGA29300.016.6058032
TCTAGCG18500.016.428
CGGACCA29950.016.3689489
CTAGCGG19200.016.09114629
TAAACGC13250.015.91698128
AGACGGA31650.015.6650876
CGCAATA20100.015.55472836
GCAAGAC34850.015.3945483
AGAGCGA32400.015.188271515
CGAAAGC32450.015.16486919
GCGAAAG32600.015.1518418
AGCGAAA32450.015.10785817
GAGCGAA33050.015.001513516
GCGCAAG32450.014.9938361
GTAAACG13700.014.98905127
TCGTTTA19950.014.92982530
Produced by FastQC (version 0.11.5)