FastQCFastQC Report
Fri 29 Apr 2016
ERR1042864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6585929
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC213580.3242974529485514No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG203670.30925022119127005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169490.25735169632105054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152600.23170611162069923No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA117150.17787923313476353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115860.1759205117455715No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA96870.14708631083025644No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG94940.14415582068983737No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT90070.13676126784846906No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC89960.13659424509435192No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT69920.10616573607155498No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT66760.10136762786237141No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66710.1012917084286818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG22600.016.944695
GACGGAC22350.016.3892617
ACGGACC22850.016.0306348
CGGACCA24050.015.230779
AGACGGA25100.015.1832686
CGCAAGA24500.015.1775512
CAAGACG27750.014.7333344
GCGCAAG26350.014.6736241
TCTAGCG17000.014.36470628
CGAGCCG20750.014.2650615
TCGTTTA16450.014.17021230
CTAGCGG17650.013.62606229
GCAAGAC31000.013.4870983
CGATCGT1102.4591267E-413.45454513
TTACTCG5700.013.30701719
GAGCGAA27750.013.13333316
CGGTCCA18400.013.07065110
CGAAAGC27850.013.01974919
GCGAAAG27850.013.01974918
AGCGAAA27950.012.97316617
Produced by FastQC (version 0.11.5)