FastQCFastQC Report
Fri 29 Apr 2016
ERR1042863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553053
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT573330.874905177785072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT461520.7042824161501516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323470.4936172498528548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC230940.3524158892046196No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG211720.323086048594449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170840.260702912062515No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA130110.1985486764718674No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG100030.1526464077125578No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT88100.13444115284890873No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG82010.12514777463267884No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG80150.12230940296072686No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT79890.12191264132916367No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC67150.10247132138256779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA4950.018.31313126
CGTACTA5000.017.7629
ACCGTAC5200.017.07692127
AAGACGG23800.016.7121855
CAAGACG25100.016.215144
TCTAGCG12850.015.83657628
ACTAGAT6000.015.72532
GACGGAC24250.015.6391767
ACGGACC24950.015.3486988
CTAGCGG13250.015.21886829
CGGACCA25650.015.0019499
CCGTACT5800.014.99138128
CTAGATC6150.014.73983833
CGCAAGA26250.014.5885722
CGCAATA14000.014.53571536
AGACGGA26400.014.5056826
GTACTAG6200.014.32258130
GCGCAAG27200.014.2830891
GCAAGAC31550.014.2488113
TAGACCG1304.451358E-614.2307685
Produced by FastQC (version 0.11.5)