FastQCFastQC Report
Fri 29 Apr 2016
ERR1042862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7315924
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC737521.0081023258306128No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG660380.9026610992678437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT578460.7906861799001739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT467430.6389213447269272No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA396790.542364846873751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT325790.44531627173819743No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG295580.4040227864586893No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG235520.32192789318205056No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT232520.3178272491622384No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG229490.3136855987022282No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC207710.2839149231183921No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC203660.2783790536916458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171540.2344748250528573No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT156730.21423131240838478No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA154400.21104647888633069No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC143530.19618847872121142No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC135110.18467933783893872No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT120500.16470920146245369No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT106300.14529948643534296No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG103920.142046308846292No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT103790.14186861427210015No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG102070.13951757836740786No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG92060.12583509615463473No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG89130.12183013382861822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC88330.12073662875666832No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA87950.12021721384749212No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC81860.11189290648727351No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC79350.10846203432403069No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT78270.10698580247689835No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG77970.10657573807491712No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA77160.10546856418956786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG26400.017.028415
ACGGACC27700.016.2292428
TCTAGCG17950.016.18105728
CGGACCA27900.015.9802879
GACGGAC28100.015.8665497
CTAGCGG18450.015.84281829
CAAGACG31250.015.3924
CGCAAGA29250.015.369232
AGACGGA29550.015.1505926
GTACTAG4250.014.7999991
GCGCAAG31200.014.34935951
TCGCTAA1551.2129203E-714.32258114
GCGAAAG30900.014.30906118
CGGTCCA21250.014.27764610
TTAACGG2600.014.23076935
CGCAATA20700.014.21014536
CGAAAGC31250.014.20819
AGCGAAA31900.013.97648817
GAGCGAA32050.013.7956316
CCGGTCC21800.013.7477069
Produced by FastQC (version 0.11.5)