FastQCFastQC Report
Fri 29 Apr 2016
ERR1042861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5429982
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT348660.6421015760273239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319220.5878840850669487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230920.42526844472044295No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC221930.40871222040883376No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG190150.35018532289793963No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA116010.2136471170622665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100400.18489932379149693No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT88470.16292871689077423No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG83720.15418098991856694No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA78800.1451201864020912No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC73600.13554372740093798No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG67120.12360998618411627No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66380.12224718240318293No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT63630.11718270889295766No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC62970.11596723525050358No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA60960.11226556552121167No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT60390.1112158382845468No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG59110.10885855606887831No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC56630.10429132177602063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG22900.018.8231455
ACGGACC22700.018.4185038
CGGACCA23650.018.0697679
CGCAAGA24000.018.03752
GACGGAC23500.017.8702137
CAAGACG25450.017.6640474
TCTAGCG13550.017.61254728
CTAGCGG13800.017.1594229
CGCGATT650.001580959117.07692329
AGACGGA25250.016.9247536
GCGCAAG26450.016.7164481
CGCAATA14900.016.1409436
CGAGCCG16250.015.25538415
CGGTCCA15950.015.19435810
GCGAAAG27750.015.13333318
GCAAGAC31900.015.0203773
GAGCGAA28600.014.81293816
CAGAGCG28600.014.81293814
AGCGAAA28550.014.70928217
CGAAAGC28850.014.6204519
Produced by FastQC (version 0.11.5)