FastQCFastQC Report
Fri 29 Apr 2016
ERR1042860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751094
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT238580.4148428107765236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230550.4008802499141902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT169750.2951612336713676No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC116670.20286575041200855No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG103000.17909635975346605No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT83180.14463335149799325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70230.12211589655811574No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA62430.1085532596059115No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA62350.10841415563717094No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT60920.1059276721959335No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA58970.10253701295788245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG20550.016.5644785
GACGGAC19500.016.412827
ACGGACC19600.016.3290828
CTAGCGG13500.015.48518629
TCTAGCG13650.015.4505528
CAAGACG22400.015.3616084
CGGACCA20900.015.224889
CGCAAGA21650.015.1247122
CGAGCCG18150.014.98347115
TAAACGC8200.014.43902428
CGCAATA14500.014.41724236
AGACGGA22400.014.3705376
GTAAACG8300.014.265058527
TCGTTTA14850.014.2020230
GCGCAAG23650.014.1585631
TACCGTC11900.014.1470597
GCGAAAG23350.013.78586818
AGCGAAA23450.013.72707817
GAGCGAA23200.013.71551716
CGTCGTA12300.013.68699210
Produced by FastQC (version 0.11.5)