FastQCFastQC Report
Fri 29 Apr 2016
ERR1042859.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042859.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5661179
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT193260.34137765295886247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170040.3003614618085738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126790.2239639481457838No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC94590.16708533681764878No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG86780.1532896239458247No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT83400.1473191361728714No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT67270.11882683801377769No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA65080.11495838587686417No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC59280.10471317017179636No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA59140.10446587186167405No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC57030.10073873304483041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC11350.015.97356828
GTAAACG11400.015.74122827
TCTAGCG14550.015.51202728
CGCTTCG12200.015.31557432
AAGACGG21050.015.2921615
CTAGCGG14900.015.14765129
CGAGCCG18950.015.03430115
CGCAATA15350.014.94462536
GACGGAC20550.014.7639917
ACGCTTC12300.014.73983831
TTCGGGC12750.014.36470635
GCTTCGG13450.014.30483233
ACGGACC21150.014.2576838
CAAGACG22950.014.1873644
AAACGCT13050.014.17624529
CTAGATA11850.014.0506333
CGCAAGA22350.013.9888152
TAGACCG1203.3048593E-513.8750015
TACCGTC13600.013.8749997
AGACGGA22750.013.8241766
Produced by FastQC (version 0.11.5)