FastQCFastQC Report
Fri 29 Apr 2016
ERR1042858.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042858.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5569522
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT234870.42170584836544317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204320.36685374436082663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT150600.2704002246512358No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC107620.19323022693868522No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG95940.172258947895349No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87270.15669208237259857No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA71790.12889795569530024No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC65300.11724525013098074No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT64850.11643728133222205No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA61420.11027876359946151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.10774712803001765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC56430.10131928736433755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG12200.017.89344228
TCTAACG1054.805461E-717.6190472
CTAGCGG12900.016.92248229
AAGACGG20800.016.8990385
GACGGAC20050.016.8852887
CGAGCCG17100.016.76900715
ACGGACC20700.016.6231888
CGCAAGA21600.015.9305542
CGGACCA21700.015.8571439
CTAACGC1059.355934E-615.8571423
CGCAATA13650.015.58608136
CAAGACG23350.015.2912224
AGACGGA22350.015.2304266
GTACTAG3250.014.81
AGGCCCG21000.014.62380910
TCGCTAA1152.2127517E-514.47826114
TATACTG9150.014.1530055
CGGTCCA15950.014.1504710
GTATTAG20400.014.14705851
GCGCAAG24850.014.0704231
Produced by FastQC (version 0.11.5)