FastQCFastQC Report
Fri 29 Apr 2016
ERR1042857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6754680
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT357700.5295587651820663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT298370.4417233680944175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT208530.3087192879603475No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC119650.17713644465762998No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG104250.15433743715468384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.13153842965173776No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87450.12946579260601537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG22350.016.2237135
ACGGACC21450.016.2144538
GACGGAC21700.016.1129047
AGACGGA22700.015.4030846
TCTAGCG14250.015.059648528
CGGACCA23400.014.8632489
TAGACCG1252.9637376E-614.7999995
CGCAAGA24450.014.4519422
CTAGCGG15100.014.21192129
CAAGACG29250.013.914534
GCGCAAG26800.013.8751
CGAGCCG20350.013.81818215
CGCAATA15300.013.78431336
ACACGCT21200.013.438689
GCGAAAG25750.013.43495218
CGTCGTA16700.013.40419110
TACCGTC17550.013.3874657
GAGCGAA26350.013.2694516
CGAAAGC26000.013.23461619
AGCGAAA26450.013.14933817
Produced by FastQC (version 0.11.5)