FastQCFastQC Report
Fri 29 Apr 2016
ERR1042856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863093
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256100.5266195814063189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217340.44691721914427707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT157150.3231482515345686No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC78020.16043287677204612No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG70490.14494890391773302No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT69520.14295428855668604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60930.12529063293669276No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA57500.11823750851567098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT53090.10916920568864301No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA52190.10731853164231077No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC50160.1031442335155836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC16500.015.9212138
GACGGAC16650.015.8888897
TCTAGCG11900.015.85714228
CTAGCGG12400.015.516129529
AAGACGG17800.015.3820225
ATTACGC855.3685793E-415.2352943
CGGACCA17750.015.1126769
TAACGGC3700.015.00000136
TTAACGG3600.014.90277835
TCGTTTA12200.014.55737730
CGCAAGA19300.014.2823822
AGACGGA18900.014.0952396
TAAACGC10400.014.05288528
GTAAACG10150.014.03448327
CGCAATA13750.013.85818236
CGTTTAT13150.013.50570431
GCGCAAG21350.013.4309131
ACGCTTC10900.013.40825731
TAACGCC13300.013.3533844
CGTAAGC1254.8506758E-513.322
Produced by FastQC (version 0.11.5)