FastQCFastQC Report
Fri 29 Apr 2016
ERR1042855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5663989
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270750.47801999615465357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT239280.42245844757113754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174420.307945513312261No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT87880.1551556685579721No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA74240.1310737008846592No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC69210.12219303392008707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64790.1143893464482364No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA61620.10879258416638875No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT57630.10174807895989911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88200.018.7726751
TCTAGCG10800.017.30092628
CTAGCGG11100.017.16666629
AAGACGG21500.015.6604665
GACGGAC21200.015.0094337
CGAGCCG17550.014.75783415
ACGGACC21850.014.6475978
CGACGTT2059.276846E-1114.43902514
TATACTG8800.014.2954545
TCGTTTA15150.014.287127530
CGCAAGA24200.013.9132232
CGCAATA13450.013.89219436
CGAACGC2009.858923E-1013.87499930
CGGACCA23300.013.8154529
TACCGTC15700.013.7866247
TACTCGC2700.013.70370420
AGACGGA23500.013.6978726
CAAGACG27050.013.6783744
TAGCGGC14250.013.50175530
CGGTCCA14700.013.46598610
Produced by FastQC (version 0.11.5)