FastQCFastQC Report
Fri 29 Apr 2016
ERR1042854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209725
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291130.4038018093616608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT255250.3540356948427298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178940.24819254548543807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101250.017.3945671
TCTAGCG14000.016.25357228
CTAGCGG14350.016.24390229
CGCAATA14800.015.536
ACGGACC18500.014.48
GACGGAC19100.014.2382217
CGGTCCA17750.013.96619810
GCGAATC5650.013.75221333
TAGCGGC17100.013.73976630
AAGACGG21150.013.2080385
GTAAACG7250.013.01379327
CCGGTCC18800.012.9893629
GTACTAG3900.012.8076931
TTAGACT6800.012.7867654
GCAATAC18300.012.73770537
GTATCAA139600.012.5630382
CGGACCA21300.012.5070429
TACCGTC18850.012.4641917
GCGAAAG21450.012.41958118
AGACGGA22350.012.4161086
Produced by FastQC (version 0.11.5)