FastQCFastQC Report
Fri 29 Apr 2016
ERR1042853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9793008
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC884530.903226056794807No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG767860.7840900364831725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT679590.6939542988221801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT591090.603583699717186No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA482400.4925963503756966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT441220.4505459405322655No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG341250.3484629033285789No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC263890.2694677672069705No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG263840.269416710371318No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG252000.25732645168879675No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT248400.2536503595218139No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC227380.2321860658134865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198370.2025628897678834No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA179980.1837841856148795No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA177170.1809147914512068No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC166960.17048898561095835No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT159490.16286109436446902No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT155870.1591645794632252No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC144900.14796270972105813No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG135440.13830275641559775No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT135000.1378534562618554No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC132840.1356478009616657No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA108100.11038487868078939No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG107850.1101295945025267No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG107690.10996621262843857No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT106670.10892465318112679No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG102010.10416615609831015No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT99380.10148056654298658No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC98030.10010203198036803No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC97950.10002034104332398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG22000.018.528
AAGACGG32850.018.359215
CTAGCGG22800.017.76973729
GACGGAC34700.016.793957
ACGGACC35100.016.6025648
CGGTCCA25800.016.34883710
CGGACCA37050.016.028349
CGCAATA25750.016.0213636
AGACGGA38500.015.5688316
CGCAAGA38800.015.4961342
GCAATAC27400.015.12408737
TCGTTTA26100.014.88505730
GCGCAAG41600.014.6754811
CCGGTCC29400.014.6615659
CAAGACG44100.014.5566884
ACGCTTC17800.014.44662931
TAAACGC17950.014.42896928
TTTAGCG5050.014.28712926
CGAGCCG28050.014.18003615
GAGCGAA42100.014.14964316
Produced by FastQC (version 0.11.5)