FastQCFastQC Report
Fri 29 Apr 2016
ERR1042852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8315773
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327740.3941185022727292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT271840.3268968501184436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT212240.2552258220612804No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC179410.21574662992845042No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG150460.18093326982350286No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT139130.16730855928847504No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA108480.13045089133625942No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT101620.12220150790551883No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA101370.12190087439856764No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG93270.11216034877334916No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA92560.11130654961360778No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC87690.10545020889819864No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87310.10499324596763285No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG85560.10288881141897453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG33200.017.4412655
TCTAGCG17450.017.17478428
CTAGCGG17950.016.69637929
ACGGACC34750.016.0776988
CGCAAGA35200.016.0298292
GACGGAC35300.015.6699717
CAAGACG39150.015.5938694
CGCAATA19900.015.33919736
CGGACCA37450.015.1655559
AGACGGA38700.014.819126
CGAGCCG28200.014.49822715
TTAACGG4350.014.4597735
TAAACGC14850.014.32659928
GCGCAAG38600.014.2823841
GCGAAAG39850.014.02007518
GAGCGAA40150.014.00747316
GCAAGAC46000.013.9956523
TAGCGGC22800.013.9561430
AGCGAAA39800.013.94472517
TCGTTTA23750.013.94315730
Produced by FastQC (version 0.11.5)