FastQCFastQC Report
Fri 29 Apr 2016
ERR1042851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8201645
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT713900.8704351383167644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT535230.6525885965559348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT429650.5238583235436306No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC224120.2732622540965867No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG197430.24072000190205745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166850.20343479875073842No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA123770.15090875062258852No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA110190.13435109663975944No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC101660.12395074402757009No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT95820.1168302212543947No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA87990.10728335596090785No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG86760.10578365681518768No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG84170.10262575373598831No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG82550.10065054022699105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT11150.025.05381237
CATCTCG15600.018.61858736
AAGACGG26700.015.7284645
TAACGGC4100.014.43902436
GACGGAC27650.014.3182647
ACGGACC27700.014.29241858
CGAGCCG21150.013.99527315
TATACTG12850.013.82101255
CGGACCA29000.013.7155179
TCTAGCG16900.013.68343328
CCGAGCC43150.013.63383514
CGCAAGA30500.013.4049182
AGACGGA30300.013.3712876
GTATTAG30900.013.3511331
CCCACGA22250.013.05393219
TTAACGG4550.013.01098835
TACCGTC21900.013.0091327
GCGCAAG31750.012.99371
CTAGCGG17550.012.96581229
CGAGCCC22650.012.90507715
Produced by FastQC (version 0.11.5)