FastQCFastQC Report
Fri 29 Apr 2016
ERR1042850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773604
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379120.487701714674429No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC357550.4599539672975366No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG328820.42299556293322893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT295850.38058280303447406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224400.28866919385139767No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA188590.24260304486824902No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG144900.18640002758051477No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT114050.14671444544898352No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG111970.1440387238660472No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC110040.14155596297418804No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT103320.13291132401393227No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG102700.1321137531574801No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC97370.1252572166012058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93600.12040747123213376No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT88250.11352520658371587No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA88120.11335797398478234No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG79060.10170314824372323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG26200.016.381685
GACGGAC26550.015.8870057
ACGGACC27100.015.7011078
TCTAGCG18000.015.31388928
CGCAAGA28400.015.1126762
CTAGCGG17750.014.90422429
CGGACCA28950.014.6977559
TACCGTC20400.014.6911767
ACCGTCG20200.014.4702988
AGACGGA30050.014.2212986
CAAGACG32400.014.1604944
ATACCGT23000.013.9956516
CGCAATA19050.013.98425236
CGTCGTA20650.013.97578610
GCGCAAG31550.013.8383511
TATACTG9200.013.6739135
CCGTCGT21500.013.5093029
TCGTTTA21250.013.40705930
TAACGGC4600.013.27173936
TAAACGC12350.013.18218628
Produced by FastQC (version 0.11.5)