FastQCFastQC Report
Fri 29 Apr 2016
ERR1042849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8333339
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325490.3905877344003406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT257950.30953978951294314No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC245730.29487579948445636No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG229410.2752918128015673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT199640.23956783709387078No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA128180.1538158954051911No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT126580.15189589671079023No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG103520.12422391552773743No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT100760.12091191777989592No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA90790.10894792591541037No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA87750.10529992839604868No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC86740.10408792922020813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG31600.017.7974685
GACGGAC31750.017.0141747
ACGGACC32100.016.828668
TCTAGCG19700.016.34010128
CGGACCA33050.016.232989
CTAGCGG19500.016.22307629
AGACGGA34700.015.7809816
GTATACG2700.015.7592591
CAAGACG37950.015.3069834
CGCAAGA36400.014.8914832
CGCAATA23200.014.67241436
TACCGTC23150.014.2246237
CGAGCCG26700.014.2041215
TAAACGC14650.014.14334528
TTCGGGC14750.014.04745835
GCGAAAG38300.014.00783318
CGAAAGC38800.013.97036119
ACCGTCG22550.013.9467848
ATACCGT24950.013.8657316
CGGTCCA23850.013.80712810
Produced by FastQC (version 0.11.5)