FastQCFastQC Report
Fri 29 Apr 2016
ERR1042848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10176327
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT744020.7311282351677575No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC578710.5686825904867248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT564850.5550627451338779No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG540930.5315572111627309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT428270.4208492907116683No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA303170.29791692031908956No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG237050.23294259313797602No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG190280.18698298511830447No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC179300.1761932375011141No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG175190.17215445219085432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172800.1698058641393894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT171190.16822376089133143No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC163610.1607751008787355No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT135160.1328180590108789No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA118800.1167415315958302No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT114480.11249638499234546No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT109680.10777955543291799No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC107060.10520495263173048No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA103780.10198178576612171No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA103290.10150027608193016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG20900.016.0215328
AAGACGG32400.015.9305555
GACGGAC32750.015.4213737
CTAGCGG21950.015.17084329
ACGGACC33750.014.9096318
GCGAATC8650.014.75722633
GGCGAAT9300.014.52150532
CGCAAGA35350.014.4964642
CGAGCCG30850.014.15235115
TTTAGCG6950.014.10791526
ATCCGTA7350.014.09523912
CGGACCA36550.014.0711359
CGCAATA25300.013.82015836
AGACGGA37200.013.77553756
CGGTCCA24350.013.75153910
CCGAGCC43500.013.69425314
TACTCGC6800.013.60294120
TACCGTC24600.013.5365857
CGTATTA7250.013.524137515
TCGTTTA26050.013.42226530
Produced by FastQC (version 0.11.5)