FastQCFastQC Report
Fri 29 Apr 2016
ERR1042847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243505
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1485251.4499431591042324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1192841.1644842268344673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT818170.7987207503681601No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC571950.5583538056553885No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG550740.5376480023195186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387330.37812252739662844No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA308400.30106882361066845No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG253470.24744460026133636No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG200530.1957630713315413No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG199120.1943865893558894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT191940.1873772697919316No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC167300.16332300321032694No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC165480.16154626761054933No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA142640.13924921206169177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT138480.13518810211934296No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA134960.13175177832197085No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT133980.13079507453747521No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA128420.12536724490298973No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT124060.12111088929033567No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC123190.12026157062450792No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC113840.1111338355377383No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC105550.10304090250358641No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT102930.10048318422258787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG36100.015.4252085
CTAACGC2800.015.1964283
TCTAGCG20850.015.17266228
GACGGAC35550.014.9353047
ACGGACC35350.014.9151348
CTAGCGG21600.014.90277829
CAAGACG41350.014.3168084
CGCAAGA36950.014.16914752
CTAGATA17950.014.0167123
CGCAATA23500.014.01276536
TTCGGGC14450.013.95501735
CGGACCA38250.013.9294119
GCGCAAG39600.013.8751
GTAAACG14600.013.81164427
AGACGGA39400.013.6637056
TCTAACG3400.013.6029422
TCGTTTA24150.013.48240230
TCTAGAT20700.013.1376812
TAAACGC15500.013.12903128
CGGTCCA25600.013.08007810
Produced by FastQC (version 0.11.5)