FastQCFastQC Report
Fri 29 Apr 2016
ERR1042846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9510846
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT426390.4483197393796514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC392030.4121925641525475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT363770.38247911910254884No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG325640.3423880483397586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281990.29649307748227655No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA212040.222945466680882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT151940.15975445296874746No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG144880.15233134886213068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129670.13633908066643075No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG120200.1263820274242691No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA115590.1215349297002601No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC113100.1189168660705893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT112680.11847526497642796No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG108500.11408028265834605No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT108020.11357559569359024No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA98510.10357648520436563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG34750.018.7395695
CTAGCGG20500.017.86829229
GACGGAC34650.017.8326117
TCTAGCG20450.017.7310528
ACGGACC35600.017.4087078
CGCAAGA36850.017.1194022
CGGACCA37200.016.6599469
AGACGGA37900.016.498686
CAAGACG40250.016.4546594
CGCAATA22900.015.99563436
GCGCAAG40600.015.6748771
CGAGCCG31050.015.133656515
TAGCGGC25250.014.87326830
TCGTTTA24550.014.69450130
CGGTCCA25500.014.654901510
GTATTAG33950.014.4948451
GCAAGAC48700.014.4353193
CCGGTCC26000.014.1596169
GCGAAAG43950.013.97497218
TATACTG13250.013.9622645
Produced by FastQC (version 0.11.5)