FastQCFastQC Report
Fri 29 Apr 2016
ERR1042845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10141130
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT929610.9166729940351814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT721990.7119423575084828No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC669280.6599659012358584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG597380.5890665044230771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT520190.5129507263983402No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA356350.35139082133845045No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG272870.26907257869685136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231110.22789373570795365No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG213820.21084435363711934No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG204440.20159489129909586No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT198660.19589532921873595No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC182910.18036451559145775No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC177480.17501008270281518No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA134140.13227322793416513No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126750.1249860715719057No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC122140.12044022707528648No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT121390.11970066452160658No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC107450.10595466185720921No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT105720.10424873756672086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG19350.016.34883728
AAGACGG30400.016.1266445
CTAGCGG20200.015.75247629
GTAAACG13400.015.32462627
GACGGAC30550.015.2602297
TAACGGC6100.015.16393436
ACGGACC31600.014.8702538
TTAACGG6100.014.86065535
CGGTCCA22400.014.53571410
CGCAATA22400.014.53571436
CGAGCCG28300.014.51236715
CGCAAGA32900.014.3951382
TAAACGC15200.014.36184228
CGGACCA32950.014.3171469
AGACGGA34500.013.9956536
ACGCTTC15650.013.83067131
CAAGACG39250.013.5745224
CCGGTCC24850.013.251519
TCGTTTA22600.013.01548730
TAGCGGC25250.012.74851430
Produced by FastQC (version 0.11.5)