FastQCFastQC Report
Fri 29 Apr 2016
ERR1042844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7923122
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT748050.944135405210219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT557160.7032076497118182No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC512890.647333210317852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG428190.5404309058979528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT359260.4534323717342735No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA275920.34824656240305274No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG187000.23601807469328379No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG159760.2016376877700482No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG157400.1986590639396945No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156810.19791440798210602No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT150140.18949600927513172No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC149500.1886882468804595No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC125520.15842239965508545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT97120.12257794339150653No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA94430.11918281707639994No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86660.10937607675358274No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82130.10365863355379357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC20900.017.6148348
TCGTTTA18050.016.70637330
TAACGCC18500.016.74
TCGCCGG18050.016.6038817
CTAGCGG14300.016.1713329
TCTAGCG14250.016.09824628
ATCGCCG18800.016.03989416
CGGACCA22500.016.0333339
AACGCCG19350.015.9664095
CGCATCG18850.015.89920413
AAGACGG23350.015.8458245
ATCGTTT19400.015.54381529
CCGGTCG19150.015.45691920
GACGGAC23550.015.3184717
TGGTCGG20000.015.1737
GCATCGC20150.015.14888414
CGCAAGA23850.015.1257872
CGAGCCG22650.015.11037515
ATGGTCG19850.015.09823736
AATAACG20400.014.9632352
Produced by FastQC (version 0.11.5)