FastQCFastQC Report
Fri 29 Apr 2016
ERR1042843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293463
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT552230.5364861174514349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT454420.4414646460574056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321850.3126741700047885No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC205720.19985499534996143No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG186310.18099836760476043No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT133180.1293830851677419No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128280.12462278243969012No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA108590.10549413739574329No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT104950.1019579125120477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1452.9885996E-916.5862065
AAGACGG34000.016.2691155
GACGGAC35200.015.188927
ACGGACC35350.015.0721368
TCTAGCG25350.015.03353128
CTAGCGG25600.014.81445329
CGTATTA7900.014.75316415
TCTATAC13400.014.4962683
CGCAAGA36950.014.4695542
CGGACCA38100.013.9842529
CGCAATA27350.013.86654536
AGACGGA39450.013.7870716
CGGTCCA30050.013.60565910
ATCCGTA8300.013.59638612
GCGCAAG42000.013.5226181
TTAACGG7150.013.45454635
GTAAACG19050.013.40157427
CCGGTCC30550.013.3224229
ACGACGG29200.012.9880136
CGACGGT29100.012.9690737
Produced by FastQC (version 0.11.5)