FastQCFastQC Report
Fri 29 Apr 2016
ERR1042842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9598142
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328610.34236834587360765No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC286860.29887034386446876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT267000.2781788391961694No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG261650.2726048437291301No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195860.20406032750922No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT150860.1571762534873937No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA149120.15536340262521642No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT116680.12156519459703763No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG116580.12146100776587804No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA108280.11281350077963007No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC101460.10570795889454439No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG97230.10130085593649271No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA96310.10034233708982425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG37150.017.0309565
TCTAGCG23650.016.7399628
CGCAAGA38850.016.3809532
ACGGACC37800.016.003978
CTAGCGG24650.015.98580129
CAAGACG43000.015.8325594
GACGGAC38750.015.7070977
CGGACCA39050.015.6338049
GCGCAAG43300.014.782911
CGAGCCG33750.014.690371515
CGCAATA26800.014.63432936
AGACGGA42350.014.4155856
TAAACGC18150.014.37190128
GTAAACG18350.014.11444127
CGGTCCA28700.013.92334510
TAGCGGC29100.013.73195830
GCAAGAC50900.013.7023583
TCGTTTA27400.013.63868630
GCGAAAG44950.013.62291318
AGGCCCG37800.013.55687810
Produced by FastQC (version 0.11.5)