FastQCFastQC Report
Fri 29 Apr 2016
ERR1042841.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042841.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9085414
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT736350.8104748996578472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT572350.6299657891208921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT417160.4591535399487574No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC414210.4559065772897085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG390640.429963895976562No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA231200.25447381924478074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205210.22586752788590592No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG183090.20152081126958002No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG146080.16078518821486834No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT139600.1536528770180423No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG135980.14966846860253147No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT123820.13628437845540115No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC117510.12933918036096098No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC109170.12015963169097192No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT92010.10127221500308076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA8000.017.57499926
ACCGTAC8600.016.7790727
ATCTCGT8800.016.39772837
AAGACGG34650.016.3910545
ACTAGAT8400.016.29761932
ACGGACC34000.016.1058838
GACGGAC34500.016.0333337
CCGTACT8550.016.01169628
CGGACCA36050.015.4466029
CGCAAGA36500.015.408222
TCTAGCG20250.015.16543228
GTACTAG9350.014.83957330
AGACGGA37650.014.8393096
TACCGTC22650.014.7836657
ACCGTCG22550.014.5210648
CTAGCGG21600.014.30324129
CGTCGTA22200.014.2510
CCGTCGT22550.014.19290359
GCGAAAG39000.014.13589818
ATACCGT25750.013.9378646
Produced by FastQC (version 0.11.5)