FastQCFastQC Report
Fri 29 Apr 2016
ERR1042840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9948789
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT581670.5846641234425618No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC563640.5665413147268477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG525360.5280642699327526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT443030.44531047949654984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT312250.31385729459133166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA304430.30599704144896434No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG243340.2445925830771966No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT205110.20616579565613463No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG189580.19055585559207255No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT180610.18153968286994526No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG175640.17654409999046114No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC164990.16583927953442373No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC162170.1630047636953603No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT156590.1573960408648731No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA138340.13905209970781368No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138010.13872040104579564No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC137350.13805700372175952No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG130770.1314431334306115No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT120100.12071821002536089No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA116170.11676798050496397No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC116040.11663731133507807No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA113770.11435562659937808No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG113220.11380279549601463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA112040.11261672149243492No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG111130.1117020373032336No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG110220.11078735311403227No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC108520.10907860243090893No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC103740.10427399756895035No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC99600.10011268708181467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG48250.017.5606215
GCGAATC8600.016.7790733
CAAGACG50500.016.6683184
TCTAGCG31900.016.58620628
GGCGAAT9150.016.57923332
ACGGACC51050.015.9089138
CTAGCGG33750.015.8414829
GACGGAC51150.015.8054737
CGCAAGA51650.015.7241052
CCGAGCC52900.015.63232514
GACTAAG9400.015.54787226
CGGACCA53200.015.4050769
CGAGCCG43550.015.37772715
AGACGGA53300.015.3761726
CGCAATA34600.015.29190836
GTTCAAA49300.014.709941
GCGCAAG57300.014.5933691
GCGCAAT37400.014.34491935
AGGCCCG47550.014.31756110
TAGCGGC37100.014.3113230
Produced by FastQC (version 0.11.5)