FastQCFastQC Report
Fri 29 Apr 2016
ERR1042839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180910
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT457850.7407485305561803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT383070.6197631093156185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258370.41801288159834066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129430.20940282256172635No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT99980.16175611681775015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC74860.12111485202017179No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA70510.11407705337887139No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT66240.10716868551718113No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG64590.10449917568772235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC23900.017.2615078
GACGGAC23900.017.029297
CGGACCA25000.016.5769
AAGACGG25950.016.4682085
TCGTTTA17300.016.04046230
CAAGACG27150.016.0128924
CGCAAGA26200.015.8167932
TCTAGCG14600.015.45890428
AGACGGA27050.015.388176
CCGGTCG17950.014.73816220
GCGCAAG28750.014.6713051
GAGCGAA28400.014.59154916
GCGAAAG28650.014.46422318
TAAACGC13550.014.335793528
TAACGCC19650.014.31043154
CTAGCGG15800.014.2848129
CGAAAGC29000.014.225862519
CGCATCG18950.014.15567313
CAGAGCG29850.014.13065214
CGCAATA16450.014.0577536
Produced by FastQC (version 0.11.5)