FastQCFastQC Report
Fri 29 Apr 2016
ERR1042838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9232282
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT403280.4368150799553133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354820.3843253488140852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270310.29278785028446924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115020.1245845826633112No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102680.11121843981802115No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC99650.10793647767691672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG15800.016.97784829
TCTAGCG16400.016.35670928
CGCAATA17900.015.29608836
GACGGAC26600.015.0921067
AAGACGG28750.014.7356525
TCGTTTA19200.014.64583430
ACGGACC27050.014.635868
TAAACGC18450.014.43902528
AGACGGA28900.014.33916
CGGACCA28000.014.1392859
GTAAACG18450.013.9376727
TTCGGGC19100.013.75392635
GGTATCA179400.013.7254741
TACCGTC17550.013.598297
CGCAAGA29150.013.5814762
CGAGCCG23850.013.57442415
CGGTCCA21000.013.56666710
GTATTAG29800.013.4093951
CAAGACG33250.013.2977454
ACGCTTC19850.013.23425731
Produced by FastQC (version 0.11.5)