FastQCFastQC Report
Fri 29 Apr 2016
ERR1042837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9675938
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT536830.5548092598361006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT439020.45372345296135635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320140.33086197947940554No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC252730.26119431521781145No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG222780.23024124379465846No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT140390.14509187636382126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137830.14244613803850334No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA133910.1383948512278603No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA114480.11831411073531062No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA102430.10586053775871651No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG101800.10520943809272032No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT101590.10499240487072158No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC97150.10040370246274831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG36950.017.3734785
ACGGACC35450.016.9083218
GACGGAC35650.016.8134637
CGGACCA36700.016.2820159
TCTAGCG21900.015.96575328
TCGTTTA24900.015.67670730
CAAGACG42100.015.5997634
AGACGGA39150.015.5938696
CGCAAGA39450.015.42839052
CTAGCGG23050.015.16919829
CGCAATA23900.014.70711336
GCGCAAG42600.014.6784041
TAACGCC29350.014.119254
TATACTG13400.014.0820885
GCAAGAC50350.014.0724943
TAGGTCG4100.013.98780521
CGAGCCG27950.013.96601115
TACCGTC25300.013.8201587
TTAACGG3350.013.80596935
CAGAGCG44400.013.58333314
Produced by FastQC (version 0.11.5)