FastQCFastQC Report
Fri 29 Apr 2016
ERR1042836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7941092
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1049161.3211784978690588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT688880.8674877460178022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT538110.6776272079457082No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC453290.570815701417387No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG379590.4780073068036486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280630.3533896849450932No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA241720.30439138597059445No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG159970.20144584649063377No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG150230.18918053083883174No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG139010.1750514916588298No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT128830.16223209603918454No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC127580.16065800522144813No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA109880.13836887924230068No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC108830.13704664295540211No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT103740.13063694514557947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.1223005601748475No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA96510.12153240385579213No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.11902645127395578No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA82840.10431814667302684No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA81770.10297072493304446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG15900.022.57232923
TATCTCG16050.022.36136836
ATCTCGT16450.021.48024237
CCCACGA17700.020.79943519
ACGGACT17400.020.62643827
CACGAGA18600.019.79301321
TCTCCGA19600.019.06632611
CGGACTC19150.019.0313328
ATCTCCG19800.018.87373510
CCTATCT19850.018.546634
GCCCACG19700.018.4060918
CCACGAG20500.018.31951120
CGAGCCC20450.018.18337415
TCCTATC20500.017.86829233
GAGACGG21400.017.8084124
CCGAGCC37900.017.47493414
CTATCTC21050.017.3135435
TCCGAGC21600.017.1296313
TCTAGCG16500.017.04242328
ACTCCTA21500.017.03720931
Produced by FastQC (version 0.11.5)