FastQCFastQC Report
Fri 29 Apr 2016
ERR1042835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8553077
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT497950.5821881411800689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399940.4675978013526594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281010.32854842765942593No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC158500.18531342579986126No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG143210.1674368183520387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123520.14441586343721682No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT97400.11387714620130276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC25650.015.9395718
CTAACGC3400.015.7794123
GACGGAC27000.015.1425937
TCTAGCG16000.014.79999928
CGGACCA27100.014.7453889
CTAGCGG16200.014.61728429
TATACCG4950.014.5757585
AAGACGG28400.014.5264085
CGCAATA17300.014.0086736
AGACGGA29200.013.7482886
CGCAAGA29750.013.6806722
GTACTAG7400.013.51
CAAGACG33400.013.3488034
CGAGCCG21800.013.32339515
CCGAGCC34750.013.25611614
TCTAACG3500.013.2142852
TAACGCC21800.013.1536694
GTCTAAC5350.013.1401861
TAGACCG1153.581366E-412.8695655
GCGCAAG33400.012.8502991
Produced by FastQC (version 0.11.5)