FastQCFastQC Report
Fri 29 Apr 2016
ERR1042834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7675376
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT677680.8829274292230114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT479130.6242430338266164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT373550.48668625484927386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC218700.28493718092768355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG194510.25342080961245417No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173360.22586515631286336No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT131800.17171797186222537No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA116800.1521749553376929No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA106540.1388075320349127No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT101020.13161570195388475No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA97850.12748561112836687No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG88670.11552528501535299No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC86760.11303680757789585No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG85890.11190331261947298No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC83520.10881551600859685No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA82500.10748659088492864No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG81830.10661366948016618No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG79160.10313501253879939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA4950.016.44444526
TCTAGCG19150.015.93994728
CGAGCCG27600.015.68478315
CTAGATA19700.015.6827423
TCTAGAT21150.015.657212
AAGACGG36050.015.5492375
CTAGCGG19800.015.41666729
CGTACTA5200.015.29807729
CAAGACG37550.015.2729684
ACGGACC35900.014.9958228
CCGAGCC34650.014.94949414
GACGGAC36200.014.8715477
ACCGTAC5500.014.827
CGGACCA37150.014.5410519
CGCAATA21100.014.46682436
CGCAAGA37900.014.3509242
CGGTCCA22350.014.31991110
AACCTCG22550.014.1108649
CCGTACT5950.013.99159728
AGACGGA38900.013.9820056
Produced by FastQC (version 0.11.5)