FastQCFastQC Report
Fri 29 Apr 2016
ERR1042833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671097
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT925380.9568511204054722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT689520.7129697902936968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT501330.5183796626173846No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC258680.2674774123349192No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG241060.24925817619242163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212280.21949940115376776No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT185940.19226360773757104No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT140220.14498872258235027No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA139210.14394437363207088No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA131810.1362927080557666No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA130350.13478305511773897No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC118330.12235426860055275No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG117440.12143400071367291No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC116460.12042067202924342No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG112910.11674994057034069No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA109180.11289308751633864No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG106760.11039078607111479No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG106420.11003922305814946No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC103330.10684413567561156No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG102490.10597556823181485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT8250.017.26666632
AAGACGG47750.016.0397915
GCGAATC8600.015.91860433
CAAGACG49250.015.8517764
CGCAATA32200.015.79968936
CTAGCGG31650.015.7819929
CGCAAGA48900.015.7004082
ACGGACC47800.015.6746868
GACGGAC47600.015.6628167
TCTAGCG31750.015.55748228
GACTAAG8950.015.50279326
CGGACCA49950.015.2222219
CGAGCCG36100.015.220221515
TAAACGC17200.014.84302328
GCAAGAC56050.014.7537923
CCGAGCC47000.014.68191514
GCGCAAG52500.014.6590481
AGACGGA51400.014.5408556
GCAATAC35200.014.50568237
GCGCAAT35300.014.46458935
Produced by FastQC (version 0.11.5)