FastQCFastQC Report
Fri 29 Apr 2016
ERR1042832.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042832.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809242
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243350.3573819229805608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242990.3568532297721244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT164140.24105473120209267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78440.11519637574931249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1107.821491E-716.81818212
TTAACGG14400.015.67361135
TAACGGC14700.015.35374136
GGGGTTA11350.014.1806176
GGTATCA127800.014.0993751
TAATACT20950.013.7756574
ATAGGGG11150.013.4394623
GGGTTAG12350.013.3319847
TAGGGGT11650.013.1802584
ATTAGAG19200.013.1041663
TTTAACG18250.012.97534234
TCTAATA19500.012.712822
TATTAGA21650.012.56122
GTATTAG25350.012.5522681
CTCTAAT18100.012.4696131
AACGGCC19050.012.2362237
AATACTG23550.012.1762215
CTAATAC25850.012.1663453
GGTAAAC17000.011.97058935
GTTTAAC20050.011.90274233
Produced by FastQC (version 0.11.5)